Does masturbation decrease testosterone?

Masturbation itself does not decrease testosterone levels; rather, it is the frequency of masturbation that might influence hormonal changes. According to Andrew Huberman, frequent masturbation, particularly when it involves ejaculation, can increase prolactin levels which may in turn reduce libido and inhibit the release of hormones like LH that can increase testosterone. Frequent masturbation, defined as daily or multiple times per day, could potentially be detrimental to the hormone profile of a male 1.