What is hydrogel tissue chemistry?


Hydrogel tissue chemistry, as discussed by Dr. Karl Deisseroth and , involves creating a gel-like substance inside the cells of a tissue or even an entire organism simultaneously. This gel serves as a scaffold, allowing scientists to physically anchor molecules such as proteins and RNAs, which are precursors to proteins, to this structure. The process involves removing unwanted components like lipids using detergents, which makes the tissue transparent while retaining the important molecules in their original positions within the cells. This methodology enhances the accessibility and tractability of the tissue for research, providing clearer insights into its structure at the cellular and subcellular levels. The first developed form of this technology was called "Clarity," which is still widely used along with variants developed later 1.

Clear Brains

Karl explains the concept of hydrogel tissue chemistry and the clarity method, which allows scientists to make brains transparent and anchor interesting molecules in place. Andrew shares his experience of looking through a cleared brain.

Huberman Lab

Dr. Karl Deisseroth: Understanding & Healing the Mind | Huberman Lab Podcast #26