NADH and Glycolysis

A buildup of NADH poses a challenge for glycolysis, as NAD is essential for the process to occur. Reduction of NAD to NADH is crucial, but it must be reversed to replenish NAD levels. Without sufficient NAD, glycolysis halts, and while converting pyruvate to lactic acid or ethanol can help, it results in minimal energy yield and is not sustainable without oxygen.